Product Description:

Trypsin, an animal-derived product, is the most commonly used enzyme for harvesting cells in culture. Trypsin is a pancreatic serine protease (proteolytic) enzyme with specificity for peptide bonds involving the carboxyl group of the basic amino acids, Arginine Lysine. Trypsin often contains a crude mixture of lipases, nucleases, polysaccharides proteases extracted from Porcine pancreas.

Most cell cultures grow as a single thickness cell layer sheet attached to a substrate known as a monolayer. When subculturing adherent cells, these intercellular cell-to-substrate links connections must be gently dissociated. Proteolytic enzymes such as Trypsin (i.e. a serine peptidase), breaks gently separates these bonds by creating a single-cell suspension from which new subcultures are realized.

Trypsin solutions are widely utilized as cell dissociation reagents for continuous cell culture of adherent growing cells. Trypsin proteolysis trypsinization is a process in which proteins have been digested treated with Trypsin are thus said to be trypsinized. Biological Industries' Trypsin is designed not only to gently dissociate cells from almost any support substrates but also as well as from each other in order to actualize cell manipulation techniques in addition for other studies that require intact cell-surface proteins. Trypsin, as a solution, is available in a varied array of formulations with without EDTA. EDTA is a chelator that binds calcium magnesium ions that may otherwise inhibit the Trypsin activity which then hydrolyzes gains access to the intercellular bonds being cell-cell / cell-substrate bonds. Crude Trypsin is often the subculturing agent of choice for cell dissociation/disaggregation of adherent cells although the treatment may be cytotoxic if prolonged. Over-trypsinization is a common cause of subculture problems.

The use of Crude Trypsin often involves multiple changes the variability among lots can dramatically influence the effectiveness of the dissociation process. Regarding the use of Crude Trypsin, some important facts must be noted: 

v Cells must NEVER remain in the Crude Trypsin for longer than 3-5 minutes as they may be seriously damaged in the process (i.e. damage to the intracellular proteins).

v Cells should NEVER be left without a fluid layer

v Do not permit prolonged growth on culture ware as the cells will be very difficult to remove(i.e. after 5-7 days)

产品官网链接：https://www.bioind.com/worldwide/trypsin-edta-solution-a-0-25-edta-0-02/

说明书下载：胰酶Trypsin EDTA Solution A (0.25%), EDTA (0.02%)说明书