EINECS 编号 233-140-8
Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Frequently Asked Questions are available for this Product.
磷酸钙转染试剂盒包含:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES 缓冲盐 (H1012)
25 ml 分子生物学级纯水(W4502)
Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:
BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D
? Suitable for transient and stable transfection
? Reproducible for a wide range of cell types
? Widely referenced
? Inexpensive
磷酸钙法转染是将DNA引入真核细胞的常用方法。这一
The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.
该操作方案要求缓慢混合含有磷酸钠的HEPES缓冲盐
grade | for molecular biology |
sterility | sterile; 0.2 μm filtered |
form | solution |
usage | 1 kit sufficient for 160 transfections (6 cm dishes) |
1 kit sufficient for 400 transfections (3.5 cm dishes) | |
1 kit sufficient for 80 transfections (10 cm dishes) | |
shipped in | dry ice |
storage temp. | ?20°C |
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894