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Calcein-AM BioReagent, suitable for fluorescence, ≥96.0% (HPLC)

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号:17783-1MG

牌:Sigma 西格玛

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别名: Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester Calcein acetoxymethyl ester
  • 经验分子式(希尔表示法) C46H46N2O23

  • 分子量 994.86

  •  MDL number MFCD05861516

  •  PubChem Substance ID 24850694

产品名称

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

Calcein-AM has been used in flow cytometry.

General description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.


性质

product line   BioReagent
assay   ≥96.0% (HPLC)
fluorescence   λex 496 nm; λem 516 nm in 0.1 M Tris pH 8.0, esterase; Ca2+
suitability   suitable for fluorescence
storage temp.   ?20°C

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