Components

Ingredients
KH₂PO₄, 1 g/l
(NH₄)₂SO₄ 5 g/l
MgSO₄, 0.5 g/l
NaCl, 0.1 g/l
CaCl₂, 0.1 g/l
inositol, 2 mg/l

Application

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.8g yeast nitrogen base powder , 5 g glucose, and 5-10 mg of appropriate amino acid in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.