Description

5'			G ↓	A	A	T	T	C		3'
3'			C	T	T	A	A ↑	G		5'

Thermo Scientific FastDigest EcoRI restriction enzyme recognizes G^AATTC site cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.

Thermo Scientific FastDigest EcoRI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, multiple DNA digestion within 5–15 minutes eliminating any need for buffer change subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation incubation times for this other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases  T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features

• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.