Thermo Scientific KpnI restriction enzyme recognizes GGTAC^C sites cuts best at 37°C in its own unique buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, heat inactivation for this other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: Asp718I.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control industry leading manufacturing process • Convenient color-coded Five Buffer System • Includes universal Tango buffer for double-digestions • BSA premixed in reaction buffers • Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning • Restriction site mapping • Genotyping • Southern blotting • Restriction fragment length polymorphism (RFLP) • SNP
Note: For methylation sensitivity, refer to product specifications.
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