Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
• Blunting of DNA ends: fill-in of 5'-overhangs / removal of 3'-overhangs(see References1, 2) • Blunting of PCR products with 3'-dA overhangs • Synthesis of labeled DNA probes by the replacement reaction(see Reference3) • Oligonucleotide-directed site-specific mutagenesis(see Reference4) • Ligation-independent cloning of PCR products
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