Description
Thermo Scientific RNase A, DNase protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C U residues.
It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.
Highlights
• RNase A is free of DNase activity. It is not necessary to heat it before use.
Applications
• Plasmid genomic DNA preparation
• Removal of RNA from recombinant protein preparations
• Ribonuclease protection assays. Used in conjunction with RNase T1
• Mapping single-base mutations in DNA RNA
Notes
Recommended concentration of RNase A is 1 to 100 µg/mL depending on the application. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M higher, RNase A specifically cleaves single-stranded RNA.
描述
Thermo Scientific RNase A，不含DNase及蛋白酶，是一种内切核糖核酸酶，可特异性降解单链RNA的C及U残基部位。
它可以剪切核苷酸5'-核糖与相邻的嘧啶核苷酸3'-核糖上所连磷酸基团间的磷酸二酯键。获得的2', 3'-环状磷酸盐可进一步水解为相应的3'-核苷磷酸。
产品特点
• RNase A不含DNase活性。在使用前无需进行加热。
应用
• 质粒及基因组DNA的制备
• 重组蛋白制备中去除RNA
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