An E. coli strain that carries the cloned SrfI gene from Streptomyces sp. (T.G. Simcox)
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
CutSmart® Buffer | -20 | 10X |
One unit is defined as the amount of enzyme required to digest 1 µg of pNEB193-SrfI DNA in CutSmart incubated for 1 hour at 37°C in a total reaction volume of 50 µl.
1X CutSmart® Buffer
Incubate at 37°C
1X CutSmart® Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C
NEBuffer 1.1: 10%
NEBuffer 2.1: 50%
NEBuffer 3.1: 0%
CutSmart® Buffer: 100%
-20°C
300 mM NaCl
10 mM Tris-HCl
1 mM DTT
0.1 mM EDTA
500 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
65°C for 20 min
CpG Methylation: Blocked
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