An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story)
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
CutSmart® Buffer | -20 | 10X |
Gel Loading Dye, Purple (6X) | 25 | 6X |
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
1X CutSmart® Buffer
Incubate at 37°C
1X CutSmart® Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C
NEBuffer 1.1: 10%
NEBuffer 2.1: 10%
NEBuffer 3.1: 10%
CutSmart® Buffer: 100%
-20°C
10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
65°C for 20 min
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
1. BbsI-HF has the same specificity as BbsI (NEB #R0539), but it has been engineered for reduced star activity.
2. Star activity may result from a glycerol concentration of > 5%.
3. BbsI is an isoschizomer of BbvII.
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