An E. coli strain that carries the cloned and modified ApoI gene from Arthrobacter protophormiae (C. Polisson).
The following reagents are supplied with this product:
Store at (°C) | Concentration | |
CutSmart® Buffer | -20 | 10X |
Gel Loading Dye, Purple (6X) | 25 | 10X |
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
1X CutSmart® Buffer
Incubate at 37°C
1X CutSmart® Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C
NEBuffer 1.1: 10%
NEBuffer 2.1: 100%
NEBuffer 3.1: 10%
CutSmart® Buffer: 100%
-20°C
10 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C
80°C for 20 min
CpG Methylation: Not Sensitive
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