An E. coli strain that carries the cloned and modified ApoI gene from Arthrobacter protophormiae (C. Polisson).

Reagents Supplied

The following reagents are supplied with this product: 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Reaction Conditions

1X CutSmart® Buffer
Incubate at 37°C

1X CutSmart® Buffer:
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 μg/ml BSA
pH 7.9 @ 25°C

Activity in NEBuffers

NEBuffer 1.1: 10%
NEBuffer 2.1: 100%
NEBuffer 3.1: 10%
CutSmart® Buffer: 100%

Diluent Compatibility

• Diluent B

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
200 μg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 min

Methylation Sensitivity

CpG Methylation: Not Sensitive 

Companion Products

• ApoI
• Gel Loading Dye, Purple (6X)

Materials Sold Separately

• CutSmart® Buffer