DescriptionBack to Top

Highlights

• Isolated from a recombinant source
• 3' → 5' single strand exonuclease
• Supplied with 10X Reaction Buffer

Product Source

Reagents Supplied

The following reagents are supplied with this product:

Advantages FeaturesBack to Top

Applications

• Exonuclease I degrades excess single-stranded primer oligonucleotide from a reaction mixture containing double-stranded extension products

Properties UsageBack to Top

Unit Definition

Reaction Conditions

1X Exonuclease I Reaction Buffer
Incubate at 37°C

1X Exonuclease I Reaction Buffer:
67 mM Glycine-KOH
6.7 mM MgCl₂
10 mM β-ME
pH 9.5 @ 25°C

Usage Concentration

10,000 units/ml

Storage Temperature

Storage Conditions

10 mM Tris-HCl
100 mM NaCl
5 mM β-ME
0.5 mM EDTA
100 μg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

80°C for 20 min