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Qiagen 凯杰 Biosharp Omega

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Separase Antibody 50ul

价:
1250.00
价:
¥1250.00

号:AF9195

牌:Affinity

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周一至周五:9:00-18:00

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 产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Sheep(80%), Rabbit(90%), Dog(90%)
克隆:
Polyclonal
特异性:
Separase Antibody detects endogenous levels of total Separase.
RRID:
AB_2843385
引用格式: Affinity Biosciences Cat# AF9195, RRID:AB_2843385.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

 展开/折叠

Caspase like protein ESPL1; Caspase-like protein ESPL1; ESP 1; ESP1; ESPL 1; Espl1; ESPL1_HUMAN; Extra spindle poles like 1; Extra spindle poles like 1 protein; Extra spindle poles-like 1 protein; FLJ46492; KIAA0165; Separase; Separin; Similar to fission yeast cut1and gene; SSE;

 抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
        10        20        30        40        50        60        70        80        90       100       110       120       130       140       150       160       170       180       190       200       210       220       230       240       250       260       270       280       290       300       310       320       330       340       350       360       370       380       390       400       410       420       430       440       450       460       470       480       490       500       510       520       530       540       550       560       570       580       590       600       610       620       630       640       650       660       670       680       690       700       710       720       730       740       750       760       770       780       790       800       810       820       830       840       850       860       870       880       890       900       910       920       930       940       950       960       970       980       990      1000      1010      1020      1030      1040      1050      1060      1070      1080      1090      1100      1110      1120      1130      1140      1150      1160      1170      1180      1190      1200      1210      1220      1230      1240      1250      1260      1270      1280      1290      1300      1310      1320      1330      1340      1350      1360      1370      1380      1390      1400      1410      1420      1430      1440      1450      1460      1470      1480      1490      1500      1510      1520      1530      1540      1550      1560      1570      1580      1590      1600      1610      1620      1630      1640      1650      1660      1670      1680      1690      1700      1710      1720      1730      1740      1750      1760      1770      1780      1790      1800      1810      1820      1830      1840      1850      1860      1870      1880      1890      1900      1910      1920      1930      1940      1950      1960      1970      1980      1990      2000      2010      2020      2030      2040      2050      2060      2070      2080      2090      2100      2110      2120
MRSFKRVNFGTLLSSQKEAEELLPALKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATLRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRAVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDKAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQMYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVDSCKSTVVWMLEALEGLSGQELTDHMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDSLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPEETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLDSVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASSLKHLDQTTDTYLLLSLTCDLLRSQLYWTHQKVTKGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNNLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLNQPSNESLQKVLQSGLKFVAARIPHLEPWRASLLLIWALTKLGGLSCCTTQLFASSWGWQPPLIKSVPGSEPSKTQGQKRSGRGRQKLASAPLRLNNTSQKGLEGRGLPCTPKPPDRIRQAGPHVPFTVFEEVCPTESKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEPKRRGTASRGRGRARKGLSLKTDAVVAPGSAPGNPGLNGRSRRAKKVASRHCEERRPQRASDQARPGPEIMRTIPEEELTDNWRKMSFEILRGSDGEDSASGGKTPAPGPEAASGEWELLRLDSSKKKLPSPCPDKESDKDLGPRLRLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEIADQLQGLSLQEMPGDVPLARIQRLFSFRALESGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLHLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQPERAQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

 种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
 
90
Dog
 
90
Sheep
 
80
Pig
 
0
Horse
 
0
Bovine
 
0
Xenopus
 
0
Zebrafish
 
0
Chicken
 
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

 翻译修饰 - Q14674 作为底物

Site PTM Type Enzyme
S3 Phosphorylation  
K64 Ubiquitination  
Y140 Phosphorylation  
K153 Ubiquitination  
K277 Ubiquitination  
K299 Ubiquitination  
K313 Ubiquitination  
K324 Ubiquitination  
K491 Ubiquitination  
K562 Acetylation  
K562 Ubiquitination  
K567 Ubiquitination  
K593 Ubiquitination  
Y719 Phosphorylation  
K722 Ubiquitination  
K755 Ubiquitination  
K760 Ubiquitination  
S810 Phosphorylation  
Y973 Phosphorylation  
K1008 Ubiquitination  
K1016 Ubiquitination  
K1020 Ubiquitination  
S1073 Phosphorylation  
K1083 Ubiquitination  
T1111 Phosphorylation  
S1122 Phosphorylation  
T1123 Phosphorylation  
S1126 Phosphorylation P06493 (CDK1) , P28482 (MAPK1)
S1153 Phosphorylation  
K1189 Ubiquitination  
T1210 Phosphorylation  
K1252 Ubiquitination  
S1305 Phosphorylation  
K1309 Ubiquitination  
S1316 Phosphorylation  
K1322 Ubiquitination  
S1325 Phosphorylation  
K1336 Ubiquitination  
R1341 Methylation  
T1346 Phosphorylation  
T1363 Phosphorylation P53350 (PLK1)
S1396 Phosphorylation  
S1399 Phosphorylation P53350 (PLK1)
S1444 Phosphorylation  
S1455 Phosphorylation  
S1475 Phosphorylation  
S1501 Phosphorylation P17612 (PRKACA) , Q9UQM7 (CAMK2A)
S1508 Phosphorylation  
S1513 Phosphorylation  
K1518 Ubiquitination  
S1528 Phosphorylation  
S1538 Phosphorylation  
S1539 Phosphorylation  
S1545 Phosphorylation  
S1552 Phosphorylation  
Y1614 Phosphorylation  
S1649 Phosphorylation  
S1660 Phosphorylation  
K1692 Ubiquitination  
K1732 Ubiquitination  
K1747 Ubiquitination  
S1752 Phosphorylation  
K1762 Ubiquitination  
S1768 Phosphorylation  
S1769 Phosphorylation  
T1771 Phosphorylation  
K1796 Ubiquitination  
K1803 Ubiquitination  
S1820 Phosphorylation  
K1832 Ubiquitination  
T1884 Phosphorylation  
S1887 Phosphorylation  
S1889 Phosphorylation  
K1901 Ubiquitination  
K1994 Ubiquitination  
K2103 Ubiquitination  
Y2104 Phosphorylation  
S2118 Phosphorylation  

 研究背景

功能:

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

翻译修饰:

Autocleaves. This function, which is not essential for its protease activity, is unknown.

Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.

细胞定位:

Cytoplasm. Nucleus.
 

Extracellular region or secretedCytosolPlasma membraneCytoskeletonLysosomeEndosomePeroxisomeERGolgi apparatusNucleusMitochondrionManual annotationAutomatic computational assertionSubcellular location
亚基结构:

Interacts with PTTG1. Interacts with RAD21.

 研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

 

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