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| Positive WB detected in | HepG2 cells, LNCaP cells, pig heart tissue, HEK-293 cells, HeLa cells, HuH-7 cells, L02 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in |
human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
| APPLICATION | DILUTION |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
| WB | See 66 publications below |
| IHC | See 1 publications below |
| IF | See 23 publications below |
| CoIP | See 1 publications below |
66305-1-Ig targets Vinculin in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | FC, IF, IHC, WB, ELISA |
| Cited Applications | CoIP, IF, IHC, WB |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | Human, Mouse, Rat, Hamster, Pig, Zebrafish |
| Immunogen | Vinculin fusion protein Ag24946 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | vinculin |
| Synonyms | CMD1W, Metavinculin, MVCL, VCL, Vinculin |
| Calculated molecular weight | 1133 aa, 124 kDa |
| Observed molecular weight | 117 kDa |
| GenBank accession number | BC039174 |
| Gene symbol | VCL |
| Uniprot |
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