Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) sodium pyruvate, but is often used with higher glucose levels, with without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer is certified to ISO 13485 ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer is certified to the ISO 13485