Assess enrichment of proteins in your sample using the ProteoMiner Protein Enrichment Introductory Large-Capacity Kit to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in ≥50 mg of sample. The protein complexity of the sample is maintained.
The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away.
For fractionation of the protein sample after binding to the beads, the ProteoMiner Protein Enrichment Introductory Large-Capacity Kit can be used with the ProteoMiner Sequential Elution Reagents (1633003). The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics. For larger numbers of samples the ProteoMiner Protein Enrichment Large-Capacity Kit (1633007) and Sequential Elution Reagents can be purchased together in one kit, the ProteoMiner Sequential Elution Large-Capacity Kit (1633011). Sequential elution has been optimized for samples for SELDI. Elution reagents are not compatible with direct use of fractions in 2-D gel electrophoresis.
ProteoMiner Protein Enrichment and Elution Kits | Capacity | Number of Samples | Catalog Number |
Small-Capacity Enrichment Kit | ≥10 mg | 10 | 1633006 |
Introductory Small-Capacity Enrichment Kit | ≥10 mg | 2 | 1633008 |
Large-Capacity Enrichment Kit | ≥50 mg | 10 | 1633007 |
Introductory Large-Capacity Enrichment Kit | ≥50 mg | 2 | 1633009 |
Sequential Elution Reagents | 10 | 1633003 | |
Small-Capacity Elution Kit (combines 1633006 and 2633003) | ≥10 mg | 10 | 1633010 |
Large-Capacity Elution Kit (combines 1633007 and 2633003) | ≥50 mg | 10 | 1633011 |
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