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Choose the ReadyPrep Protein Extraction Kit (Signal) to enrich for signaling proteins. This kit uses differential solubilization at 4°C in the presence of Triton X-100 to isolate proteins associated with lipid rafts.
Many proteins located within lipid rafts are resistant to solubilization at 4°C by Triton X-100 and other nonionic detergents. The ReadyPrep Protein Extraction Kit (Signal) utilizes a proprietary mixture of nonionic detergents to extract and remove hydrophilic and soluble proteins, leaving behind the signal proteins in a detergent-insoluble fraction.
Proteins that can be isolated using this kit include
For 50 extractions of 25–50 mg of cells or tissue:
Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.
| ReadyPrep Kit for Fractionation by Cellular Location | Proteins Targeted | Catalog Number |
| ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) | Cytoplasmic and nuclear | 1632089 |
| ReadyPrep Protein Extraction Kit (Membrane I) | Membrane-anchored, with one or two transmembrane domains, and hydrophobic | 1632088 |
| ReadyPrep Protein Extraction Kit (Membrane II) | More than two transmembrane domains and/or proteins resistant to Membrane I Kit | 1632084 |
| ReadyPrep Protein Extraction Kit (Signal) | Associated with lipid rafts | 1632087 |
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