Use the ReadyPrep 2-D Cleanup Kit to remove contaminants and concentrate protein samples for isoelectric focusing (IEF), 2-D gel electrophoresis, and any other application where protein sample cleanup or concentration is needed. The kit improves 2D resolution and reproducibility.

• Remove contaminants such as salts, lipids, detergents, nucleic acids and phenolic compounds
• Concentrate proteins in dilute samples
• Reduce streaking, background staining, and other artifacts
• Clean up protein samples in any form; precipitation is unaffected by the presence of chaotropes, detergents, common buffers, salts and other reagents commonly used in protein preparations
• Resuspend protein in any IEF/2DE-compatible or other rehydration/sample buffer

The TCA-like precipitation removes ionic contaminants from samples. Proteins are quantitatively precipitated and concentrated, with substances that can interfere with IEF or other applications being washed away.  Dilute samples can be concentrated for higher protein loads and enhanced spot detection. The isoelectric points of the proteins in the purified sample remain unaltered.

Product Contents

For 50 samples, each 100 µl containing up to 500 µg protein

• Precipitation agent 1, 15 ml
• Precipitation agent 2, 15 ml
• Wash reagent 1, 2 ml
• Wash reagent 2, 50 ml
• Wash 2 additive, 0.25 ml
• Instruction manual

Packaging Options

Related Products

• ReadyPrep Kits for Fractionation by Cellular Location
• ReadyPrep Kits for Fractionation by Solubility
• Aurum™ Ion Exchange Mini Kits for Fractionation by Charge
• Rotofor^® Liquid-Phase IEF Cells for Fractionation by pI
• Model 491 Prep Cell and Mini Prep Cell for Fractionation by Solubility
• ReadyPrep™ 2-D Starter Kit
• PROTEAN^® i12™ IEF System
• ReadyStrip™ IPG Strips
• ReadyPrep Reduction-Alkylation Kit (1632090)
• PowerPac™ HV High-Voltage Power Supply

Related Categories

• Protein Gels
• Electrophoresis Chambers
• 2-D Electrophoresis Buffers and Reagents
• Ampholytes
• Tube Gel IEF Systems
• Gel Imaging Systems
• 2-D Electrophoresis Workflow

Supporting Documentation

• ReadyPrep 2-D Cleanup Kit Instruction Manual
• Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure
• ReadyStrip IPG Strip Instruction Manual
• PROTEAN i12 IEF System Brochure
• Host Cell Protein (HCP) Workflow Quick Guide

Online Resources

2-D Electrophoresis and Analysis