The Bio-Plex Pro human isotyping 6-plex panel is a premixed multiplex kit for detecting the human immunoglobulins IgG₁, IgG₂, IgG₃, IgG₄, lgA, and lgM.

Kit Components

• Coupled 6.5 µm magnetic beads
• Detection antibodies
• Standards
• 1-level quality controls
• Detection antibody diluent
• Isotyping diluent — for use with samples, standards, and controls
• Assay and wash buffers
• Streptavidin-PE
• 96-well flat bottom plate
• Sealing tape
• Assay quick guide and product data sheet

Benefits

• Obtain robust results with decreased variability and increased reproducibility using the all-in-one kit format — no additional reagent kits or standards are needed
• Perform simplified plate processing during assay washing steps with Bio-Plex Pro wash stations

Other Available Human Isotyping Assays
Our human isotyping assays also include singleplex kits for IgA (171-A3101M), IgM (171-A3104M), IgE (171-A3102M), and IgG total (171-A3103M).

Multiplexing Capabilities
Due to different sample dilution considerations, the IgE assay should not be multiplexed with other assays in the isotyping product line.

Multiplexing singleplex assays (IgA, IgM, and IgG total) is not recommended due to lot differences of components. If assaying for IgA and IgM, then consider running the 6-plex panel.

To avoid cross-reactivity, the IgG total assay should not be multiplexed with any of its subclasses (IgG₁, IgG₂, IgG₃, and IgG₄).

Instrument Compatibility
Analyze your multiplex data with any of Bio-Rad’s readers:

• Bio-Plex^® MAGPIX™ multiplex reader
• Bio-Plex^® 200 system
• Bio-Plex 3D suspension array system