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Use this midi Criterion Tris-HCl Protein Gel for running either denaturing or nondenaturing PAGE. The gel does not contain SDS. For denaturing electrophoresis use standard Laemmli buffers.
Try Criterion Stain Free™ Tris-HCl Gels for visualization of proteins directly in gels or on blots.
| Gel Percentage | Optimum Separation Range | Catalog Number | ||||
| 12+2 well, 45 µl | 18 well, 30 µl | 26 well, 15 µl | Prep+2 well, 700 µl | 11 cm IPG/prep+1, 550 µl | ||
| 5% | 100–250 kD | 3450001 | 3450002 | 3450003 | – | – |
| 7.5% | 40–200 kD | 3450005 | 3450006 | 3450007 | 3450008 | – |
| 10% | 30–150 kD | 3450009 | 3450010 | 3450011 | 3450012 | 3450101 |
| 12.5% | 20–120 kD | 3450014 | 3450015 | 3450016 | 3450017 | 3450102 |
| 15% | 10 –100 kD | 3450019 | 3450020 | 3450021 | 3450022 | – |
| 18% | 10–50 kD | 3450023 | 3450024 | 3450025 | 3450026 | – |
| 4–15% | 20–250 kD | 3450027 | 3450028 | 3450029 | 3450030 | 3450103 |
| 4–20% | 10–200 kD | 3450032 | 3450033 | 3450034 | 3450035 | 3450104 |
| 8–16% | 20–120 kD | 3450037 | 3450038 | 3450039 | 3450040 | 3450105 |
| 10–20% | 10 –100 kD | 3450042 | 3450043 | 3450044 | 3450045 | 3450107 |
| 10.5–14% | 25–200 kD | 3459949 | 3459950 | 3459951 | – | 3450106 |
| Gel percentage | 18 |
| Comb | Prep+2-well |
| Sample volume, µl | 800 |
| Shelf life, months* | 3 |
| Gel storage conditions | Store flat at 2–8°C; do not freeze |
| Recommended sample buffer (Laemmli, dilute 1:1 with sample) | 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 25% glycerol, 0.01% bromophenol blue |
| Gel dimensions (W x L x thickness), cm | 13.3 x 8.7 x 0.1 |
| Cassette dimensions (W x L x thickness), cm | 15.0 x 10.6 x 0.53 |
| Cassette material | Styrene copolymer |
| Comb material | Polycarbonate |
| Storage tray material | PET |
| * From date of manufacture. |
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