Use Criterion TGX Precast Protein Gels with IPG/prep wells for the second dimension after isoelectric focusing (IEF) when using 11 cm IPG strips or for preparative electrophoresis of proteins ~4–200 kD. The novel formulation TGX (Tris-Glycine eXtended) Gels have a long shelf life and can be used for either denaturing SDS protein separations or native electrophoresis.

Bio-Rad offers a wide range of Criterion TGX Precast Protein Gels, including other percentage gels with IPG/prep wells, as well as gels with a 700 μl prep well and 2 reference wells.

Run 1–2 4 midi gels in 35 minutes using the Criterion Cell. For higher throughput, the Criterion Dodeca Cell can run up to 12 gels.

Features and Benefits

• Can be used for denaturing electrophoresis with standard running buffer containing SDS
• Laemmli-like separation in the presence of SDS
• Run time as short as 20 min
• Proteins retain native conformation and activity in the absence SDS
• IPG well accommodates 11 cm ReadyStrip™ IPG Strips
• 12 month shelf life

Related Products

For faster gel and western blotting analyses, use Criterion™ TGX Stain-Free™ Precast Protein Gels.

• 2x Laemmli Sample Buffer (1610737)
• Native Sample Buffer (1610738)
• 10x Tris/Glycine/SDS Running Buffer (1610732)
• 10x Tris/Glycine Running Buffer (1610734)
• Protein Ladders and Standards (Markers) including IEF and 2-D Standards

Supporting Documents

Criterion Precast Gels Application Guide, Instruction Manual