小鼠脂多糖/内毒素(LPS)ELISA Kit 48T

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小鼠脂多糖/内毒素(LPS)ELISA Kit 48T

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Qiagen 凯杰 Biosharp Omega

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首页分子生物学试剂武汉华美Cusabio >

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市场价：: 2500.00

促销价：: ¥2500.00

货号：CSB-E13066m

品牌：武汉华美Cusabio

批量采购：单个商品金额满20,000.00元，即可向客服咨询相关优惠信息

配送至：

北京朝阳

支付：账期货到付款

数量：

盒 (国产商品3-4天到货,进口商品3-4周到货,特殊商品除外)

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

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商品介绍
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Mouse LPS ELISA Kit was designed for the quantitative measurement of Mouse LPS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .
缩写：

LPS
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich
说明书：

下载最新说明书

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LPS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	98
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	96
	Range %	86-98

回收率：

The recovery of mouse LPS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-98
EDTA plasma (n=4)	94	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.424	2.522	2.473	2.406
5	2.051	1.955	2.003	1.936
2.5	1.345	1.255	1.300	1.233
1.25	0.679	0.655	0.667	0.600
0.625	0.461	0.444	0.453	0.386
0.312	0.257	0.239	0.248	0.181
0.156	0.171	0.165	0.168	0.101
0	0.069	0.065	0.067

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse LPS antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled LPS antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days