产品说明(Description)

Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed. 

Accession #: P01375

Known as: Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0.

质量控制(Quality Control)

Purity:         Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Bioactivity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

                     The ED50 for this effect is 10-50 pg/ml.

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

背景(Background)

TNFα is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease.

电泳(SDS-PAGE)

参考文献(Reference)

TBK1 Suppresses RIPK1-Driven Apoptosis and Inflammation during Development and in Aging[J]. Xu D. et al. Cell. 2018.

Therapeutic potential of C1632 by inhibition of SARS-CoV-2 replication and viral-induced inflammation through upregulating let-7[J]. Chen Xie. et al. Signal Transduction and Targeted Therapy. 2021.

Sodium iodate induces ferroptosis in human retinal pigment epithelium ARPE-19 cells[J]. Binghua Liu. et al. Cell Death & Disease. 2021.

Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-κB Signaling Pathway in Rheumatoid Arthritis[J]. Xiao-Yu Cai. et al. Frontiers in Pharmacology. 2020.

Immunotherapy of patient with hepatocellular carcinoma using cytotoxic T lymphocytes ex vivo activated with tumor antigen-pulsed dendritic cells[J]. Ying Wang. et al. Journal of Cancer. 2021.

Chaperone-mediated autophagy is involved in the execution of ferroptosis[J]. Zheming Wu. et al. PNAS. 2019.

YAP1 inhibits the induction of TNF‐α‐stimulated bone‐resorbing mediators by suppressing the NF‐κB signaling pathway in MC3T3‐E1 cells[J]. Beining Yang. et al. Journal of Cellular Physiology. 2019.

Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression[J]. Kezhou Zhu. et al. Cell death & Disease. 2018.

1, 25-dihydroxy-vitamin D3 with tumor necrosis factor-alpha protects against rheumatoid arthritis by promoting p53 acetylation-mediated apoptosis via Sirt1 in synoviocytes[J]. X Gu. et al. Cell Death and Disease. 2017.

Differential transcription Profiles of long-coding in primary human brain microvascular endothelial cells in response to meningitic Escherichia coli[J]. Ruicheng Yang. et al. Scientific Reports. 2016.