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Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome c and the leuco form of many dyes. HRP has a molecular weight of 40 kDa and an optimum pH of 7.0. Stability: HPOFF is stable for 9-12 months at 2-8°C. HPOD is stable 2 to 3 years at 2-8°C.
| Technical Notes | The RZ (Reinheitzahl) which is the absorbance ration, A403/A275, has been used as an indication of purity. However, Shannon et al., JBC, 241, 266 (1966) report that this ratio for the isozymes varies from 2.50 to 4.19. This, together with the influence exerted by buffer and pH, would seem to render questionable the preciseness of this ratio as a criterion of purity.^Numerous different methodologies are utilized for the determination of peroxidase activity. Listed below are some approximate conversions as determined by us.^ 1 Our unit = 4.6 o-dianisidine units previously used by us^ 1 Our unit = 0.62 ABTS units (μmole of dye oxidized per minute, pH 6.0, 25°C, 1.7 mM dye)^ 1 Our unit = 2 ABST units (μmole of dye oxidized per minute, pH 5.0, 25°C, 8.7 mM dye)^ 1 Our unit = 0.5 guiacol units (μmole of guiacol oxidized per minute, pH 7.0, 25°C)^1 Our unit = 0.5 pyrogallol to purpogallin unit (mg of product per 20 seconds, pH 6.0, 20°C)^ 1 Our unit = 5 pyrogallol to purpogallin units (μmole of product per minute at pH 6.0, 30°C) |
| Source | Horseradish Roots |
| Description | A soluble, dialyzed, lyophilized powder. RZ 1.0. |
| Storage | Store at -20°C or 2-8°C. |
| Activity | ≥85 units per mg dry weight |
| Unit Definition | One our Unit decomposes 1μmole of H2O2 per minute at 25°C, pH 7.0 using aminoantipyrine and phenol. |
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