概述

EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

应用

1.EDC is ideal for peptide immobilization and hapten-carrier protein conjugation. 2.Used as dehydrating agent, polypeptide condensing agent and crosslinking agent in the synthesis of polypeptides, proteins and nucleotides.

使用说明

The unstable nature of the intermediate in aqueous solutions makes 2-step coupling, however in conjunction with N-hydroxysuccinimide, a 2-step coupling is possible.

组份

1 g/5 g/25 g