Anti-GAPDH Antibody 100ul

产品概况

货号	BA2913
产品名称	Anti-GAPDH Antibody
基因名	GAPDH
抗体来源	Rabbit
克隆	Polyclonal
抗体亚型	Rabbit IgG
分子量	36KD
免疫原	A synthetic peptide corresponding to a sequence at the N-terminus of human GAPDH(30-44aa IVAINDPFIDLNYMV), identical to the related mouse and rat sequences.
内容	500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA
纯化方式	Immunogen affinity purified.
浓度	500ug/ml
产品形态	Liquid
保存条件	12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料	Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide(NAD). The enzyme is though to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase(GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.
Uniprot ID	GAPDH: P04406
推荐配套的二抗和检测试剂	Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

货号

BA2913

产品名称

Anti-GAPDH Antibody

基因名

GAPDH

抗体来源

Rabbit

克隆

Polyclonal

抗体亚型

Rabbit IgG

分子量

36KD

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human GAPDH(30-44aa IVAINDPFIDLNYMV), identical to the related mouse and rat sequences.

内容

500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA

纯化方式

Immunogen affinity purified.

浓度

500ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.

背景资料

Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide(NAD). The enzyme is though to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase(GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.

Uniprot ID

GAPDH: P04406

推荐配套的二抗和检测试剂

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

产品应用细节

为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用	稀释度*
Western blot:	1:500-2000
Immunohistochemistry(Paraffin-embedded Section):	1:50-400
Immunohistochemistry(Frozen Section):	1:50-400
Immunocytochemistry:	1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

*最优稀释度需要用户自己调试，此处数据仅供参考。

**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品