Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of downstream biochemical assays, especially those for quantification of enzymatic activity. This buffer does not contain SDS, a reagent that interferes with many activity assays.

The lysis buffer has been optimized for mammalian samples, but it can also be used for preparation of plant or bacterial samples.

The buffer just requires a simple 5-fold dilution, and minimal hands-on-time. Cell lysates are ready in only 15 minutes.