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| Product Data | |
| Description | Purified recombinant protein of Rat tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11). |
| Species | Rat |
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | PAMMEGSWLDVARRGKPEAQPFAHLTINAADIPSGSHKVSLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPADYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISVQVSNPSLLDPDQDATYFGAFKVQDID |
| Tag | Tag Free |
| Predicted MW | 19.4 kDa |
| Concentration | lot specific |
| Purity | >95% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2 |
| Bioactivity | Determined by its ability to induce NFkB in RAW264.7 cells in the absence of any cross-linking. The expected ED50 for this effect is 10.0-25.0 ng/ml. Cell culture factor (PMID: 30022569) |
| Endotoxin | Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg) |
| Reference Data | |
| Locus ID | 117516 |
| Refseq Size | 957 |
| Cytogenetics | 15q11 |
| Refseq ORF | 954 |
| Synonyms | RANKL |
| Summary | induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006] |
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