Product Data | |
Description | IFN-alpha A/D Bgl II (Universal Type I Interferon) human recombinant protein, 1 x 10^5 U |
Species | Human |
Expression Host | E. coli |
Predicted MW | 19.4 kDa |
Concentration | |
Purity | >95% Purified from E. coli by a combination of hydrophobic interaction, ion exchange and gel permeation chromatography |
Buffer | Buffer System: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
Bioactivity | Biological: Assay Used to Measure Bioactivity: Interferon was titrated in the Cytopathic Effect Inhibition (CPE) assays as listed. Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml. Mouse (L929/EMCV) – developed and performed at PBL Biomedical Laboratories. The EC50 for interferon in this assay is ~5 U/ml. Specific: x 10 units/mg (MDBK/VSV) |
Endotoxin | < 1 EU/μg |
Reference Data |
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