Product Data | |
Tissue | Lung |
Host | Human |
Lysis Buffer | Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4) |
Component | 1 vial of 100ug whole cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT) |
Storage | Shipped on dry ice. Store the lysate at -80°C and avoid repeated freeze / thaw cycles. The lysate is stable for at least one year from date of shipping when stored at -80°C. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage. |
Recommended Application | IP, WB |
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