| Product Data |
| _pt | 1 |
| expression | Mammalian |
| ecoli_selection | Ampicillin |
| availabilities_filter | In Stock |
| sub_category | Mammalian Expression Vectors |
| fpfeature | Blue, monomer |
| multiple_cloning | /assets/images/trueorf/ps100043-mcs.gif |
| expression_promoter | CMV |
| description | pCMV6-AC-mBFP, mammalian vector with C-terminal mBFP, 10ug |
| pdf_url | /datasheet/ps100043.pdf |
| product_name | pCMV6-AC-mBFP Mammalian Expression Vector |
| features | - ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mBFP (monomeric) tag.
- mBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein. mBFP possesses bright blue fluorescence with excitation/emission maxima at 402 and 457 nm, characterized by high photostability and extremely high pH-stability.
Compared to EBFP2, mBFP is more than 1.8 times brighter, much more pH-stable and has twice shorter maturation half-time at 37°C. Narrow fluorescence emission peak of mBFP provides for accurate and easy spectral separation with cyan and green fluorescent proteins and makes it a preferable tag for multicolor labeling.
Good overlap between the emission spectrum of mBFP and the absorbance spectra of mGFP allows using these two proteins as a FRET pair. - If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mBFP-tagging vector, PS100051.
- Download full vector sequence
|
| cell_selection | Neomycin |
| terms | PCMV |
| tagged_vector_tab | FP |
| name | pCMV6-AC-mBFP |
| ctag | mBFP |
| vector | Destination Vector |
| category | Vectors |
| sizes_filter | 10 ug |
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