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Qiagen 凯杰 Biosharp Omega

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pCMV6-AC-mKate Mammalian Expression Vector 10 ug

价:
6080.00
价:
¥5472.00

号:PS100039

牌:OriGene

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Specifications

Product Data
_pt 1
expression Mammalian
ecoli_selection Ampicillin
availabilities_filter In Stock
sub_category Mammalian Expression Vectors
fpfeature Far-red, monomer
multiple_cloning /assets/images/trueorf/ps100039-mcs.gif
expression_promoter CMV
description pCMV6-AC-mKate, mammalian vector with C-terminal mKate tag, 10ug
pdf_url /datasheet/ps100039.pdf
product_name pCMV6-AC-mKate Mammalian Expression Vector
features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mKate2 tag.
  • mKate2 possesses fluorescence with excitation/emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than TagFP635 and is 10-fold brighter than mPlum at physiological pH 7.5. Within the optical window optimal for light penetration in living tissues, calculated brightness of mKate2 is at least 2-fold higher compared to any monomeric fluorescent protein reported to date.
    mKate2 is characterized by complete and fast chromophore maturation at 37°C with maturation half-time <20 min (versus 40 min for mCherry). It is more photostable under both widefield and confocal illumination than other monomeric far-red proteins, including TagFP635, mRaspberry and mPlum. The high brightness, far-red emission spectrum, excellent pH resistance and photostability, coupled with low toxicity demonstrated in transgenic Xenopus laevis embryos, make mKate2 a superior fluorescent tag for imaging in living tissues.
    mKate2 is mainly intended for protein labeling. Its far-red fluorescence allows easy and reliable separation from standard green fluorescent labels in dual-color high-throughput assays.
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mKate2 -tagging vector, PS100047
  • Download full vector sequence
cell_selection Neomycin
citations Yes
terms PCMV
tagged_vector_tab FP
name pCMV6-AC-mKate
ctag mKate
vector Destination Vector
category Vectors
sizes_filter 10 ug
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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