gRNA can be produced via in vitro transcription after the target sequence being cloned
BsmB I sites are used for target sequence cloning
For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
M13 forward sequencing primer can be used to sequence the target sequence after cloning.
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