The HuSH pGFP-V-RS plasmid vector contains both 5' and 3' LTRs of Moloney murine leukemia virus (MMLV) that flank the puromycin marker and the U6-shRNA expression cassette. Upon transient transfection of the plasmids into a packaging cell line, replication deficient viruses can be obtained and used to infect target cells. The puromycin-N-acetyl transferase gene and Kanamycin gene provide selection of antibiotics puromycin and kanamycin, respectively. There is an integrated turboGFP element driven by a cMV promoter to readily verify transfection efficiency.
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