Checking the Presence of ACE Inhibition
ACE inhibitions is present in the sample if
absorbance of the sample (Total sample AU – Blank 2 AU)
is lower than Blank 1 (Total Blank 1 AU – Blank 2 AU).
For 50 tests unit size, 14 samples can be tested in triplicate
Determination of IC 50 (50% inhibitory concentration)
Prepare an inhibit ion curve using the sample concentration for
X axis and the ACE inhibitory activity for Y axis.
A typical inhibition curve is shown in Fig. 4.
Determine the concentration of the sample solution
that gives 50% ACE inhibitory activity as indicated in Fig. 4 .
For 50 tests unit size, 2 samples can be tested in triplicate
Example Assay Data
Fig. 2 Inhibition curves prepared by Alacepril and Captopril.
IC50 of Alacepril and Captopril are 3.62 μM and 2.14 nM, respectively. Both compounds are ACE inhibitors.
Fig. 3 Inhibition curves prepared by two beverages containing a valyltyrosine ( ● ) or lacto tripeptide ( ● ) .
IC50 of these beverages are 0.56% and 0.69%, respectively.
It is known that these substances have antihypertensive effects.
*Concentration of the beverage in the sample solution.
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