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Overview
| Product name | Mouse TNF-? ELISA KIT |
| Catalog no | CME0004 |
| Detection method | Colorimetric |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 3.9 pg/mL |
| Assay range | 15.625 - 1000 pg/mL |
| Assay length | 3 hours |
| Assay procedure | Multiple steps standard assay |
| Specificity | Reacts with: Mouse |
| Product information | The mouse TNF-? ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of TNF-? in mouse plasma, serum, and cell culture samples.This assay employs a quantitative sandwich enzyme immunoassay technique that measures mouse TNF-? in approximately 3 hours. A polyclonal antibody specific form mouse TNF-? has been pre-coated onto a 96-well microplate with removable strips. TNF-? in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for mouse TNF-?, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. |
| Background | |
| Tested application | Suitable for: Sandwich ELISA |
| Format | Pre-coated microplate (12 x 8 well strips) |
| Properties | |
| Storage | Store at 2 ? 8?. Please refer to protocols. |
| Components | 1 x 96 tests |
| Antibody-coated microplate | 1 unit |
| Standard (Lyophilized) | 1 x 2 vials |
| Antibody conjugate concentrate | 1 x 2 vials |
| Streptavidin-HRP solution | 1 x 2 vials |
| Standard/sample diluent | 1 bottle |
| Antibody diluent buffer | 1 bottle |
| Streptavidin-HRP diluent | 1 bottle |
| Wash Buffer Concentrate 20X | 1 bottle |
| Substrate solution | 1 vial |
| Stop solution | 1 vial |
| Adhesive Films | 4 pieces |
image of Mouse TNF-? ELISA KIT
Representative standard curve for TNF-? ELISA. TNF-? was diluted in serial two-fold steps in Sample Diluent.
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