Overview
Product name | Human IL-12p70 ELISA KIT |
Catalog no | CHE0015 |
Detection method | Colorimetric |
Sample type | Cell culture supernatant, Serum, Plasma |
Assay type | Sandwich (quantitative) |
Sensitivity | 15 pg/mL |
Assay range | 31.25 - 2000 pg/mL |
Assay length | 3.5 hours |
Assay procedure | Multiple steps standard assay |
Specificity | Reacts with: Human |
Product information | The Human IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of IL-12p70 in human plasma, serum, and cell culture samples.This assay employs a quantitative sandwich enzyme immunoassay technique that measures human IL-12p70 in approximately 3.5 hours. A polyclonal antibody specific for human IL-12p70 has been pre-coated onto a 96-well microplate with removable strips. IL-12p70 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human IL-12p70, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. |
Background | |
Tested application | Suitable for: Sandwich ELISA |
Format | Pre-coated microplate (12 x 8 well strips) |
Properties | |
Storage | Store at 2 ? 8?. Please refer to protocols. |
Components | 1 x 96 tests |
Antibody-coated microplate | 1 unit |
Standard (Lyophilized) | 1 x 2 vials |
Antibody conjugate concentrate | 1 x 2 vials |
Streptavidin-HRP solution | 1 x 2 vials |
Standard/sample diluent | 1 bottle |
Antibody diluent buffer | 1 bottle |
Streptavidin-HRP diluent | 1 bottle |
Wash Buffer Concentrate 20X | 1 bottle |
Substrate solution | 1 vial |
Stop solution | 1 vial |
Adhesive Films | 4 pieces |
image of Human IL-12p70 ELISA KIT
Representative standard curve for IL-12p70 ELISA. IL-12p70 was diluted in serial two-fold steps in Sample Diluent.
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