RBM8A RNA Binding Motif Protein 8A Human Recombinant Protein 10ug

产品概况

产品名称	RBM8A RNA Binding Motif Protein 8A Human Recombinant Protein
产品描述	RBM8A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-174 a.a.) and having a molecular mass of 20.9kDa. RBM8A is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
文献引用格式	RBM8A RNA Binding Motif Protein 8A Human Recombinant Protein (Boster Biological Technology, Wuhan, China. Catalog #PROTQ9Y5S9)
应用范围	Cell Culture
抗体来源	No

产品名称

RBM8A RNA Binding Motif Protein 8A Human Recombinant Protein

产品描述

RBM8A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-174 a.a.) and having a molecular mass of 20.9kDa.
RBM8A is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

文献引用格式

RBM8A RNA Binding Motif Protein 8A Human Recombinant Protein (Boster Biological Technology, Wuhan, China. Catalog #PROTQ9Y5S9)

应用范围

Cell Culture

抗体来源

产品详情

产品形态	Sterile Filtered colorless solution.
内容	The RBM8A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT. *可以提供无载体抗体。
储存条件	Store in -20°C for long term storage. After reconstitution, store in 4°C for short term usage within a few days. Avoid freeze-thaw cycles.

产品形态

Sterile Filtered colorless solution.

内容

The RBM8A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.
*可以提供无载体抗体。

储存条件

Store in -20°C for long term storage. After reconstitution, store in 4°C for short term usage within a few days. Avoid freeze-thaw cycles.

指标相关信息 (来源: Uniprot.org)

基因名	RBM8A
基因全名	RNA-binding protein 8A
蛋白功能	Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Tissue Specificity	Ubiquitous.
Uniprot ID	RBM8A: Q9Y5S9

基因名

RBM8A

基因全名

RNA-binding protein 8A

蛋白功能

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.

Tissue Specificity

Ubiquitous.

Uniprot ID

RBM8A: Q9Y5S9

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品