产品名称 | MAPKAPK3 Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 Human Recombinant Protein |
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产品描述 | MAPKAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 405 amino acids (1-382 a.a) and having a molecular mass of 45.4kDa. MAPKAPK3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
文献引用格式 | MAPKAPK3 Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 Human Recombinant Protein (Boster Biological Technology, Wuhan, China. Catalog #PROTQ16644) |
应用范围 | Cell Culture |
抗体来源 | No |
产品形态 | Sterile Filtered colorless solution. |
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内容 | MAPKAPK3 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 20% glycerol and 1mM DTT. *可以提供无载体抗体。 |
储存条件 | Store in -20°C for long term storage. After reconstitution, store in 4°C for short term usage within a few days. Avoid freeze-thaw cycles. |
基因名 | MAPKAPK3 |
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基因全名 | MAP kinase-activated protein kinase 3 |
蛋白功能 | Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38- alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression. |
Tissue Specificity | Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain. |
Uniprot ID | MAPKAPK3: Q16644 |
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