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Rabbit SFRP5 PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK1472-RB

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK1472-RB
    产品名称 Rabbit SFRP5 PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of rabbit SFRP5. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: CHO; Immunogen sequence: E30-H317
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <15pg/ml
    检测范围 156pg/ml-10000pg/ml
    基因名称 SFRP5
    基因全名 Secreted frizzled-related protein 5
    Uniprot ID Q5T4F7
    指标别名 Secreted frizzled-related protein 5;sFRP-5;Frizzled-related protein 1b;FRP-1b;Secreted apoptosis-related protein 3;SARP-3;SFRP5;FRP1B, SARP3;
    背景资料 Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.
    显色时间 15-20

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 749 2783 6025 877 2948 7356
    Standard deviation 53.93 178.11 439.82 74.54 256.47 698.82
    CV(%) 7.2 6.4 7.3 8.5 8.7 9.5

    Rabbit SFRP5 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000
    O.D. 0.056 0.168 0.294 0.516 0.953 1.487 1.912 2.189
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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