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Rabbit Endothelin PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0952-RB

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0952-RB
    产品名称 Rabbit Endothelin PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of rabbit Endothelin. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: rabbit peptide; Immunogen sequence: C53-W73
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <0.5pg/ml
    检测范围 3.9pg/ml-250pg/ml
    基因名称 Edn1
    基因全名 Endothelin-1
    Uniprot ID P22388
    指标别名 Endothelin-1;ET-1;Preproendothelin-1;PPET1;Edn1;
    背景资料 The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of rabbit endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.
    显色时间 15-20

    试剂盒内容

    1ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 26.2 88.7 144 25.4 91.5 151
    Standard deviation 1.18 7.94 6.81 1.73 7.05 11.93
    CV(%) 4.5 5.8 5.7 6.8 7.7 7.9

    Rabbit Endothelin PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250
    O.D. 0.068 0.093 0.121 0.193 0.354 0.715 1.559 2.457
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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