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Monkey primate TGF-Beta 2 PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0981-PR

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0981-PR
    产品名称 Monkey primate TGF-Beta 2 PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated monkey primate TGF-beta 2. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: NSO; Immunogen sequence: A303-S414
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    检测范围 31.2pg/ml-2000pg/ml
    基因名称 TGFB2
    基因全名 Transforming growth factor beta-2
    Uniprot ID P61812
    指标别名 Transforming growth factor beta-2;TGF-beta-2;BSC-1 cell growth inhibitor;Cetermin;Glioblastoma-derived T-cell suppressor factor;G-TSF;Polyergin;Latency-associated peptide;LAP;TGFB2;
    背景资料 Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured monkey primate anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
    显色时间 20-25

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 221 564 1145 236 576 1095
    Standard deviation 13.7 31.6 46.9 15.8 33.41 70.1
    CV(%) 6.2 5.6 4.1 6.7 5.8 6.4

    Monkey primate TGF-Beta 2 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25)
    Concentration(pg/ml) 0 2000 2000 2000 2000 2000 2000 2000
    O.D. 0.031 0.094 0.157 0.345 0.487 1.032 1.901 2.369
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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