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Monkey primate Neurotrophin-3 PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0472-PR

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0472-PR
    产品名称 Monkey primate Neurotrophin-3 PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate Neurotrophin-3. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: sf21; Immunogen sequence: Y139-T257
    交叉反应 There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
    敏感性 <2pg/ml
    检测范围 15.6pg/ml-1000pg/ml
    基因名称 NTF3
    基因全名 Neurotrophin-3
    Uniprot ID P20783
    指标别名 Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;NTF3;
    背景资料 Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to monkey primate chromosome 12p and monkey primate chromosome 6.1
    显色时间 20-25

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 93 357 657 146 375 732
    Standard deviation 4 17.5 34.2 7.88 22.9 47.58
    CV(%) 4.3 4.9 5.2 5.4 6.1 6.5

    Monkey primate Neurotrophin-3 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25)
    Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000
    O.D. 0.014 0.073 0.148 0.277 0.561 0.870 1.686 2.529
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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