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Monkey primate FGF1/Fgf Acidic PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0339-PR

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0339-PR
    产品名称 Monkey primate FGF1/Fgf Acidic PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate FGF1. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: E.coli; Immunogen sequence: A2-D155
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    检测范围 31.2pg/ml-2000pg/ml
    基因名称 FGF1
    基因全名 Fibroblast growth factor 1
    Uniprot ID P05230
    指标别名 Fibroblast growth factor 1;FGF-1;Acidic fibroblast growth factor;aFGF;Endothelial cell growth factor;ECGF;Heparin-binding growth factor 1;HBGF-1;FGF1;FGFA;
    背景资料 Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
    显色时间 15-20

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 83.4 288 1204 97.4 302 1357
    Standard deviation 3.67 16.13 71.04 5.90 20.54 100.42
    CV(%) 4.4 5.6 5.9 6.2 6.8 7.4

    Monkey primate FGF1/Fgf Acidic PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 2000 2000 2000 2000 2000 2000 2000
    O.D. 0.007 0.069 0.161 0.359 0.670 1.288 1.955 2.224
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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