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Qiagen 凯杰 Biosharp Omega

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Monkey primate CD56/NCAM-1 PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0706-PR

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0706-PR
    产品名称 Monkey primate CD56/NCAM-1 PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate CD56/NCAM-1. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: NSO; Immunogen sequence: L20-G718
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    检测范围 156pg/ml-10,000pg/ml
    基因名称 NCAM1
    基因全名 Neural cell adhesion molecule 1
    Uniprot ID P13591
    指标别名 Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;NCAM1;NCAM;
    背景资料 Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
    显色时间 15-20

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mea
    n(
    ng/ml) 1.52 3.48 5.34 2.06 4.16 6.27
    Standard deviation 0.082 0.212 0.368 0.140 0.312 0.508
    CV(%) 5.4 6.1 6.9 6.8 7.5 8.1

    Monkey primate CD56/NCAM-1 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000
    O.D. 0.019 0.152 0.286 0.494 0.865 1.493 2.100 2.411
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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