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Monkey primate Cadherin-2/N-Cadherin PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0669-PR

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0669-PR
    产品名称 Monkey primate Cadherin-2/N-Cadherin PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate Cadherin-2/N-Cadherin. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: NSO; Immunogen sequence: D160-A724
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <20pg/ml
    检测范围 0.78ng/ml-50ng/ml
    基因名称 CDH2
    基因全名 Cadherin-2
    Uniprot ID P19022
    指标别名 Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD325;CDH2;CDHN, NCAD;
    背景资料 Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
    显色时间 20-25

    试剂盒内容

    50ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mea
    n(
    ng/ml) 9.6 22.4 35.4 12.5 26.4 40.7
    Standard deviation 0.365 0.963 1.986 0.675 1.61 2.768
    CV(%) 3.8 4.3 5.2 5.4 6.1 6.8

    Monkey primate Cadherin-2/N-Cadherin PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 20-25)
    Concentration(ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50
    O.D. 0.025 0.075 0.119 0.210 0.369 0.749 1.355 2.007
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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