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| 货号 | EK1440 |
|---|---|
| 产品名称 | GFP PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of GFP in cell lysates. |
| 免疫原 | E.coli, S2-K238 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | 0 |
| 基因全名 | 0 |
| Uniprot ID | P42212 |
| 指标别名 | GFP|Green fluorescent protein|yfp |
| 背景资料 | The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. |
| 显色时间 | 25-30 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1440-CAP | 96-well plate precoated with anti- GFP antibody | 1 |
| EK1440-ST | Lyophilized recombinant GFP standard | 10ng/tube×2 |
| EK1440-DA | Biotinylated anti- GFP antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 39 | 220 | 444 | 38 | 224 | 429 |
| Standard deviation | 1.98 | 17.16 | 28.86 | 2.16 | 20.16 | 30.88 |
| CV(%) | 5.1% | 7.8% | 6.5% | 5.7% | 9% | 7.2% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.055 | 0.137 | 0.182 | 0.295 | 0.496 | 0.897 | 1.563 | 2.225 |
典型操作程序：
1 准备试剂和标准品
2 每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3 每孔添加100ul生物素标记抗体，37℃反应60分钟。
4 0.01M TBS洗涤3次。
5 每孔添加100ulABC，37℃反应30分钟。
6 0.01M TBS洗涤5次。
7 TMB37℃避光反应30分钟以内。
8 加入TMB终止液，读数。
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