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Chinese Hamster IGF-2 PicoKine™ ELISA Kit 96T

价:
2280.00
价:
¥2280.00

号:EK0380-HA

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0380-HA
    产品名称 Chinese Hamster IGF-2 PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Chinese hamster IGF-2. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: E.coli; Immunogen sequence: A25-E91
    交叉反应 There is no detectable cross-reactivity with IGF-1.
    敏感性 <5pg/ml
    检测范围 62.5pg/ml-4000pg/ml
    基因名称 Igf2
    基因全名 Insulin-like growth factor II
    Uniprot ID P01346
    指标别名 Insulin-like growth factor II;IGF-II;Multiplication-stimulating activity;MSA;Multiplication-stimulating polypeptide;Insulin-like growth factor II;Preptin;Igf2;Igf-2;
    背景资料 Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
    显色时间 25-30

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 478 1536 2743 453 1737 2968
    Standard deviation 20.55 87.55 126.2 30.8 140.7 222.6
    CV(%) 4.3 5.7 4.6 6.8 8.1 7.5

    Chinese Hamster IGF-2 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 25-30)
    Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000
    O.D. 0.049 0.112 0.196 0.309 0.593 1.040 1.668 2.171
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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