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Bovine FGF9 PicoKine™ ELISA Kit 96T

价:
2480.00
价:
¥2480.00

号:EK0348-BV

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK0348-BV
    产品名称 Bovine FGF9 PicoKine™ ELISA Kit
    产品描述 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Bovine FGF9. 96wells/kit, with removable strips.
    免疫原 Expression system for standard: sf21; Immunogen sequence: M1-S208
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <15pg/ml
    检测范围 62.5pg/ml-4000pg/ml
    基因名称 FGF9
    基因全名 Fibroblast growth factor 9
    Uniprot ID P31371
    指标别名 Fibroblast growth factor 9;FGF-9;Glia-activating factor;GAF;Heparin-binding growth factor 9;HBGF-9;FGF9;
    背景资料 Fibroblast growth factor-9(FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The Bovine FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208 amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%. FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.
    显色时间 15-20

    试剂盒内容

    10ng/tube
    SKU 名称 数量
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    n="3">I
    ntra-Assay Precisio
    n
    n="3">I
    nter-Assay Precisio
    n
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 256 1145 2278 247 1242 2226
    Standard deviation 11.52 43.51 76.17 17.48 80.73 135.8
    CV(%) 4.5 3.8 3.3 7.4 6.5 6.1

    Bovine FGF9 PicoKine™ ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000
    O.D. 0.087 0.097 0.115 0.145 0.247 0.500 1.026 2.188
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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